Browse RNAi Hits

Experiment Assay Cell Line Reference Hits 1 Formation of actin-based protrusions Actin staining S2R+ Kunda Patricia et al (2003) Curr Biol 13: 1867-75 9 2 JAK/STAT signaling JAK/STAT Luciferase Reporter Assay S2-NP Baeg Gyeong-Hun et al (2005) Genes Dev 19: 1861-70 491 3 Microtubule-based motor proteins Microtubule and DNA Staining S2 Goshima Gohta et al (2003) J Cell Biol 162: 1003-16 9 4 Nervous system development Neuronal staining Drosophila Embryo Ivanov Andrej I et al (2004) Proc Natl Acad Sci U S A 101: 16216-21 43 5 Cardiogenic genes Cardiac tissue staining Drosophila Embryo Kim Yong-Ou et al (2004) Proc Natl Acad Sci U S A 101: 159-64 132 6 JAK/STAT Signalling JAK/STAT Pathway Assay (6×2×DrafLuc) Kc Muller Patrick et al (2005) Nature 436: 871-5 91 7 JAK/STAT Signalling JAK/STAT Pathway Assay (4×socsLuc) Kc Muller Patrick et al (2005) Nature 436: 871-5 91 8 Mycobacterial Infection M. fortuitum Infection Assay S2 Philips Jennifer A et al (2005) Science 309: 1251-3 141 9 Mycobacterial Infection Bacterial Phagocytosis Assay S2 Philips Jennifer A et al (2005) Science 309: 1251-3 54 10 Actin-based lamella formation Actin Staining S2 Rogers Stephen L et al (2003) J Cell Biol 162: 1079-88 19 11 Growth and Viability ATP Viabilty assay Kc Boutros Michael et al (2004) Science 303: 832-5 252 12 Growth and Viability ATP Viabilty assay S2R+ Boutros Michael et al (2004) Science 303: 832-5 398 13 Innate Immune Response LPS-stimulated Imd pathway activity assay S2 Foley Edan et al (2004) PLoS Biol 2: E203 94 14 Innate Immune Response Basal Imd pathway activity assay S2 Foley Edan et al (2004) PLoS Biol 2: E203 25 15 Hh / Wg Signalling Hedgehog Luciferase reporter gene assay Clone 8 Lum Lawrence et al (2003) Science 299: 2039-45 23 18 Hh / Wg Signalling Wingless Luciferase reporter gene assay Kc Lum Lawrence et al (2003) Science 299: 2039-45 5 19 DCV Host Factors Quantification of level of viral infection S2 Cherry Sara et al (2005) Genes Dev 19: 445-52 66 20 Wnt-Wingless Signalling Wingless Luciferase reporter gene assay Clone 8 DasGupta Ramanuj et al (2005) Science 308: 826-33 234 21 Cytokinesis Quantification of multinucleate cells Kc Eggert Ulrike S et al (2004) PLoS Biol 2: e379 214 22 Terminal Cytokinesis Events Hoechst staining S2 Echard Arnaud et al (2004) Curr Biol 14: 1685-93 35 23 L. monocytogenes host factors L. monocytogenes infection SL2 Agaisse Herve et al (2005) Science 309: 1248-51 311 24 Imd Pathway Imd Pathway Reporter Assay S2 Kleino Anni et al (2005) EMBO J 24: 3423-34 29 25 Imd Pathway Act5C Promoter Activity Assay S2 Kleino Anni et al (2005) EMBO J 24: 3423-34 82 26 IMD Pathway IMD Pathway Reporter Assay SL2 Gesellchen et al (2005) EMBO Rep : 24 27 Phagocytosis FACS analysis of ingested fluorescent E.coli S2 Ramet Mika et al (2002) Nature 416: 644-8 45 28 Phagocytosis FACS analysis of ingested fluorescent S.aureus S2 Ramet Mika et al (2002) Nature 416: 644-8 44 29 Morphogenesis Cytoskeletal staining Kc Kiger A A et al (2003) J Biol 2: 27 85 30 Morphogenesis Cytoskeletal staining S2R+ Kiger A A et al (2003) J Biol 2: 27 162 31 Cell cycle kinases FACS analysis S2 Bettencourt-Dias M et al (2004) Nature 432: 980-7 97 32 Alternative splicing regulators Alternative splicing assay S2 Park Jung W et al (2004) Proc Natl Acad Sci U S A 101: 15974-9 47 33 Cell Size and Cell Cycle Progression Flow cytometry S2 Bjorklund Mikael et al (2006) Nature 439: 1009-13 452 34 Compartmentalization of an intracellular pathogen L. monocytogenes infection (LLO-minus) S2 Cheng Luisa W et al (2005) Proc Natl Acad Sci U S A 102: 13646-51 32 35 Compartmentalization of an intracellular pathogen L. monocytogenes infection S2 Cheng Luisa W et al (2005) Proc Natl Acad Sci U S A 102: 13646-51 90 36 Compartmentalization of an intracellular pathogen L. monocytogenes infection (LLO-S44A) S2 Cheng Luisa W et al (2005) Proc Natl Acad Sci U S A 102: 13646-51 8